BRUTILALA ELISA EN Kit

Specificatio

 

Test Ratio & Interpretatione

 

Gradus I test procedure

1,1 lava Solutio Praeparatio

WOrking concentration wLiquid Lingua:Before use, the wash solution should be restored to room temperature (20-25℃) and shaken evenly to make it uniform. Then dilute 20 times the concentrated wash solution with purified water (for example, 5ml concentrated washing solution is added to 95ml purified water), mix well, and the diluted washing liquid can be stored at 2-8℃for 7 days.

Sample 1.2 Praeparatio

①whole animal sanguinem capta est, centrifuged ad 4000rpm ad X minuta, et supernatant erat collecta {{II}} Serum etiam coagulated naturaliter sine hemolysis {{III}}

②When in sample mole est magna, ut ad curare experimentalem reactionem tempus est consistent . miscere in Serum sample cum antibody opus solutio ad I: I, {{V}}} {Serum et positivum} {{{V}} {Sermini et positivum} {{{V}}}}

1.3 test procedure

①Take in enzyme label micropulate (secundum numerum exempla, potest esse disassembled et usus aliquoties), ut in puteis utriusque in premixed mixtisque in enzyme et in plante, et incubant ad XXXVII gradum ad XXX minutes {{}}

②open ad signing laminam film, excutite de solutione in laminam tum, adde 300μl of working concentration lavacro liquore ad invicem bene, et abiicias super gradus V temporibus, et postremo} {{{}}

③DIlicutis enzyme conjugate:Dilute Enzyme Conjugate (L ×) L temporibus (exempli 20UL enzyme Conjugate concentrate {{III}} Ul Diluent I, in specifica tantum requiritur ratio, et posuit {{V}}

④Add 100μl of enzyme conjugate ad invicem bene, operiet laminam, et incubant ad XXXVII gradu ad XXX minuta {{III}}

⑤washing enzyme laminam, in specifica operatio est idem quod supra ② .

⑥Add 100μl subiectum solution ad invicem bene, operiet laminam et incubant ad X minuta ad XXXVII gradum ab luce {{III}}

⑦Add 50μl subsisto solution ad se bene, et determinare effectus in V minutes {{II}}

 

Gradus II praecessi interpretatione

 

De OD450 valorem uniuscuiusque bene esse legitur in {{I}} bene microplate lectorem {{II}}

2,1 Validation

 

In mediocris Od450nm valorem de positivum imperium erat <0 {{}} XXXV et mediocris Od450nm valorem negative imperium / positivum imperium erat maior quam vel aequalis III {{V}}, quod experimentalem sunt valida. Alioquin per test debet.

2.2 Calculation modum

Nam singulis sample, calculate in Pi (blockingr comedit) ut sequitur per corrigi sample et imperium values:

2.3 effectus interpretatio

Cum Pi

Cum Pi> XXX%, quod judicatur ut positivum {{I}}

Cum XX% minus quam vel aequalis ad Pi minus quam vel aequalis ad XXX%, quod est iudicatus ut suspectum, et suadeant ut repetere sampling et test una sabbatorum post {{II}}

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