Anti {- GE Porcine Pseudorabies virus antibody Elisa Kit

Specificatio

Prv - GE AB Elisa

Intendebat usum: Porcine Pseudorabies virus GE Clausus

Antibody Elisa Deprehensio ornamentum est ad determinationem GE, in Pig scriptor Serum Porcine Pseudorabies virus in Vitro. In test est ad distinguere infecta fera

Virus et vaccinated ex immunization cum GE gene deletionem vaccine.

Specimen: Serum

Materials requiritur sed non provisum:

Purificatus

Pipettor

XCVI-bene microplate lector (450nm)

Timor

Centrifuge (4000rpm)

Baptismata machina

Clibano (XXXVII gradu)

 

Test Ratio & Interpretatione

Gradus I test procedure

1,1 lava Solutio Praeparatio

WOrking concentration wLiquid Lingua:Ante usum, in lava solution restitui ad locus temperatus (20-25 gradu) et commovebitur aequaliter ad eam uniformis. Tum diluu XX temporibus in conuenerunt lavabit solution cum purificari aqua (exempli, 5ml conuenerunt baptismata solutio additur ad 95ml purificatus aqua), miscere bene, et dilutius in VII dierum.

Sample 1.2 Praeparatio

① totum animal sanguinem capta, centrifuged ad 4000rpm ad X minuta, et supernatant est collecta. Serum potest etiam coagulated naturaliter sine hemolysis.

② Dilute Serum sample I: I (addere 70UL Serum sample ad 70ul sample Diluent) et misce penitus.

Nota: negans et positivum controls opus non erit dilutius.

1.3 test procedure

①Take the enzyme label Microplate (according to the number of samples, can be disassembled and used several times), add 100ul of the diluted serum to the enzyme label Microplate, set two wells each for negative and positive samples, and add 100ul each for negative and positive controls to the reaction well respectively. Leniter agitabit in sample in bene, utendo signa laminam amet ut operias laminam et incubant ad XXXVII gradum ad LX minuta.

②open et signantes laminam film, excutite de solutione in laminam etiam, adde 300μl of working concentration baptismata liquorem ad invicem bene et abiicias liquorem in bene. Iterare super gradibus V temporibus et tandem bat aridam in mundum absorbent charta.

③Add 100μl of enzyme conjugate ad invicem bene, operiet laminam et incubant ad XXXVII gradum pro XXX minutes.

④open et signantes laminam film, excutite de solutione in laminam etiam, adde 300μl of working concentration baptismata liquorem ad invicem bene et abiicias liquorem in bene. Iterare super gradibus V temporibus et tandem bat aridam in mundum absorbent charta.

⑤Add 100μl subiectum solutio ad invicem bene, operiet laminam et incubant ad XV minuta ad XXXVII gradum a lucem.

⑥Add 50μl subsisto solutio ad invicem bene, et determinare ex eventus intra V minutes.

Gradus II praecessi interpretatione

Et OD450 valorem cuiusque bene legitur in XCVI-bene microplate lectorem.

2,1 Validation

The average OD450nm value of negative control was ≥ 0.5 and the blocking rate of positive control was >L%, ad experimentum praecessi erant validum. Alioquin per test debet.

2.2 Calculation modum

Nam singulis sample, calculate in Pi (blockingr comedit) ut sequitur per corrigi sample et imperium values:

2.3 effectus interpretatio

Cum Pi

Cum Pi> L%, quod sit iudicatus est positivum.

Cum XL% minus quam vel aequalis ad Pi minus quam vel aequalis ad L%, quod est iudicatus ut suspectum, et suadeant repetere sampling et test una sabbatorum post.

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