Bobine Virus Diarrhea Virus Antibody Elisa Kit

Specificatio

 

Test Ratio & Interpretatione

 

Gradus I test procedure

1,1 lava Solutio Praeparatio

WOrking concentration wLiquid Lingua:Ante usum, in lava solution restitui ad locus temperatus (20-25 gradu) et commovebitur aequaliter ad eam uniformis. Tum diluu XX temporibus in conuenerunt lavabit solution cum purificari aqua (exempli, 5ml conuenerunt baptismata solutio additur ad 95ml purificatus aqua), miscere bene, et dilutius in VII dierum.

Sample 1.2 Praeparatio

① totum animal sanguinem capta, centrifuged ad 4000rpm ad X minuta, et supernatant est collecta. Serum potest etiam coagulated naturaliter sine hemolysis.

② Dilute Serum sample 1:50 (adde 3UL Serum sample ad 147ul sample diluent) et misce penitus.

Nota: negans et positivum controls et opus dilute.

1.3 enzyme conjugate Praeparatio

Dilute Enzyme Conjugate I: CCL (addere 2UL enzyme conjugate (x100) ad 498ul sample Diluent) et misce penitus.

1.4 test procedure

①Take the enzyme label Microplate (according to the number of samples, can be disassembled and used several times), add 100ul of the diluted serum to the enzyme label Microplate, set two wells each for negative and positive samples, and add 100ul each for negative and positive controls to the reaction well respectively. Leniter agitabit in sample in bene, usura ad signationem laminam amet ut operias laminam et incubant ad XXXVII gradum ad XXX minutes.

②open et signantes laminam film, excutite de solutione in laminam etiam, adde 300μl of working concentration baptismata liquorem ad invicem bene et abiicias liquorem in bene. Iterare super gradibus V temporibus et tandem bat aridam in mundum absorbent charta.

③Add 100μl of enzyme conjugate ad invicem bene, operiet laminam et incubant ad XXXVII gradus ad XLV minutes.

④open et signantes laminam film, excutite de solutione in laminam etiam, adde 300μl of working concentration baptismata liquorem ad invicem bene et abiicias liquorem in bene. Iterare super gradibus V temporibus et tandem bat aridam in mundum absorbent charta.

⑤Add 100μl subiectum solution ad invicem bene, operiet laminam et incubant ad X minuta ad XXXVII gradum a lucem.

⑥Add 50μl subsisto solutio ad invicem bene, et determinare ex eventus intra V minutes.

 

Gradus II praecessi interpretatione

 

Et OD450 valorem cuiusque bene legitur in XCVI-bene microplate lectorem.

2,1 Validation

In mediocris OD450NM valorem de negans imperium erat<0.1 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥3, the experimental results were valid. Otherwise, the test should be repeated.

2.2 Calculation modum

Nam quisque sample, calculari S / P ut sequitur per corrigi sample et imperium values:

2.3 effectus interpretatio

Cum s / p<0.25, it is judged as negative.

Cum s / P major quam vel aequalis ad 0,35, quod sit iudicatus est positivum.

Cum 0,25 minus quam vel aequalis s / p<0.35, it is judged as suspicious, and suggest to repeat sampling and test one week later.

 

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